rabbit anti pde7a  (Santa Cruz Biotechnology)

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Adsorption of miR-218 by lncRNA HOTAIR regulates PDE7A and affects glioma cell proliferation, invasion, and apoptosis

doi:

Figure Lengend Snippet: Primer sequences

Article Snippet: After blocking by TBST (Beijing Solaibao Technology Co., Ltd., China) containing 5% non-fat dry milk 1 h, the membranes were incubated with primary antibody PDE7A (1:1000, ab249158, Abcam, UK) overnight at 4°C, and then incubated with secondary antibody (1:1500, ab150077, Abcam, UK) for 1 h at room temperature.

Techniques: Sequencing

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Adsorption of miR-218 by lncRNA HOTAIR regulates PDE7A and affects glioma cell proliferation, invasion, and apoptosis

doi:

Figure Lengend Snippet: HOTAIR regulates PDE7A expression in U251 cells by regulating miR-218. A. mRNA expression of PDE7A in tissues; B. mRNA expression of PDE7A in cells; C. The result of a bioinformatics prediction website regarding miR-218 and PDE7A; D. The results of a dual-luciferase reporter assay of miR-218 and PDE7A; E. Cellular protein expression level; F. Correlation analysis. Compared with NHA, *P<0.05, compared with NC group, ^P<0.05; compared with sh-HOTAIR group, %P<0.05; compared with sh-HOTAIR + miR NC group, &P<0.05.

Article Snippet: After blocking by TBST (Beijing Solaibao Technology Co., Ltd., China) containing 5% non-fat dry milk 1 h, the membranes were incubated with primary antibody PDE7A (1:1000, ab249158, Abcam, UK) overnight at 4°C, and then incubated with secondary antibody (1:1500, ab150077, Abcam, UK) for 1 h at room temperature.

Techniques: Expressing, Luciferase, Reporter Assay

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Adsorption of miR-218 by lncRNA HOTAIR regulates PDE7A and affects glioma cell proliferation, invasion, and apoptosis

doi:

Figure Lengend Snippet: The effect of miR-218 on U251 cells could be rescued by PDE7A overexpression. A. Cell proliferation; B. Cell invasion (200×); C. Apoptosis detection. Compared with the control group, #P<0.05; compared with miR-218 mimic, @P<0.05.

Article Snippet: After blocking by TBST (Beijing Solaibao Technology Co., Ltd., China) containing 5% non-fat dry milk 1 h, the membranes were incubated with primary antibody PDE7A (1:1000, ab249158, Abcam, UK) overnight at 4°C, and then incubated with secondary antibody (1:1500, ab150077, Abcam, UK) for 1 h at room temperature.

Techniques: Over Expression